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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Cancer Lett. 2007 Apr 6;254(1):75–86. doi: 10.1016/j.canlet.2007.02.013

Fig. 1.

Fig. 1

1,25D induces activation of MAPK cascades in human SaOS-2 and rat ROS 17/2.8 cells. Osteosarcoma cells were grown in the absence (-) or presence (+) of 10 nM 1,25D for 1 and 3 days. Western blots from whole cell lysates show increasing levels of phosphorylated (p-) JNK and c-Jun (A and B), and higher expression of c-Fos protein (A) in hormone-treated cell cultures. Beta-actin and vinculin were used for normalization of gel loading and transfer. Increasing levels of VDR protein were also detected over time under sustained treatment with hormone.