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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: J Surg Res. 2009 Mar 4;156(2):257–264. doi: 10.1016/j.jss.2009.02.005

FIG 3 — Effects of HIV Nef on eNOS expression in porcine pulmonary arteries. Porcine pulmonary artery rings were cultured with or without Nef for 24 h, and the total mRNA was purified from endothelial layers of the rings. The eNOS mRNA was measured with real-time PCR. (A). Nef significantly decreased the eNOS mRNA expression compared with controls (n = 4). *P < 0.05 versus controls (medium only). (B). Representative slides showed decreased eNOS immunoreactivity in the endothelium of Nef-treated porcine pulmonary arteries compared with controls. Magnification: ×400.

FIG 3 — Effects of HIV Nef on eNOS expression in porcine pulmonary arteries. Porcine pulmonary artery rings were cultured with or without Nef for 24 h, and the total mRNA was purified from endothelial layers of the rings. The eNOS mRNA was measured with real-time PCR. (A). Nef significantly decreased the eNOS mRNA expression compared with controls (n = 4). *P < 0.05 versus controls (medium only). (B). Representative slides showed decreased eNOS immunoreactivity in the endothelium of Nef-treated porcine pulmonary arteries compared with controls. Magnification: ×400.