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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Kidney Int. 2008 Aug 27;74(12):1548–1556. doi: 10.1038/ki.2008.411

Figure 2. Targeted expression of dominant-negative Gαqloop minigene to mouse parathyroid glands.

Figure 2

(a) Schematic of transgenic construct consisting of the human PTH promoter driving the expression of the dominant-negative Gαqloop minigene. (b) Genotyping of the TgPTHp–Gqloop transgenic mice by PCR. The forward and reverse primers are respectively located in the 3′ end of Gαq minigene and in the downstream poly A sequence region. Tissue-restricted expression of Gαqloop transgene by RT–PCR (c) and Western blot analysis (d).