Fig. 5.

AR is functional in clorgyline-treated cells. A firefly luciferase reporter gene driven by the super-PSA promoter is transfected into clorgyline-treated cells and control cells. A renilla luciferase reporter construct is co-transfected and its activity is used to normalize the sPSA-controlled firefly luciferase activity. PSA promoter activity is up-regulated by androgen in a dose dependent manner in clorgyline-treated cells. The induction of PSA promoter activity is blocked by Casodex, an AR antagonist. The treatment conditions are shown at the bottom of the graph. Triplicate samples are analyzed and standard deviation is calculated for each treatment. AR, androgen receptor; PSA, prostate-specific antigen.