Fig. 7.

Inhibition of MAO-A induces secretory morphology and represses the basal phenotype in E-PZ cells. (A) Shows cells grown in control medium are randomly distributed on the plate. (B) Depicts cells treated with clorgyline demonstrating the formation of acinar-like structures. The magnification for (A) and (B) is × 200. For all images, the size bar is 100 µM. (C) Western blotting analysis shows that basal cell K14 expression is greatly decreased in clorgyline-treated cells compared with control, whereas the expression of the transit amplifying/secretory cell K18 is not affected. GAPDH levels were determined as an internal control for experimental variation. The treatment conditions are shown on top of each lane. (D) Flow cytometry analysis shows that CD57 expression is significantly induced by VRT treatment, but not further enhanced by clorgyline or androgen. MAO-A, monoamine oxidase A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.