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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Cell Cycle. 2009 Jan 6;8(2):191–194. doi: 10.4161/cc.8.2.7357

Figure 1.

Figure 1

Regulation of PCNA ubiquitination by Usp1 and Rad17. (A) Depletion of Usp1 leads to elevated levels of ubiquitinated PCNA in undamaged and damaged cells. HeLa cells transfected with Usp1 siRNA or control siRNA were treated with UV or left untreated. The unmodified and ubiquitinated PCNA was detected with an anti-PCNA antibody. (B) Depletion of Rad17 leads to reduced levels of ubiquitinated PCNA in UV- and HU-treated cells. Cells transfected with Rad17 siRNA or control siRNA were treated with UV and HU as indicated, and were analyzed by immunoblotting as above.