Skip to main content
. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: J Biol Chem. 2007 Oct 5;282(48):34828–34838. doi: 10.1074/jbc.M707423200

FIGURE 3. FKBP12.6-removal does not alter the sensitivity of RyR2 to activation by caffeine.

FIGURE 3

[3H]Ryanodine binding to cell lysate prepared from HEK293 cells expressing RyR2 or expressing both RyR2 and FKBP12.6 was carried out at various caffeine concentrations (0.01–20 mM), 5 nM [3H]ryanodine, and 500 mM KCl. Panel A shows [3H]ryanodine to RyR2 (filled circles) and RyR2/FKBP12.6 (open circles). Panel B shows [3H]ryanodine binding to RyR2/FKBP12.6 in the presence (open squares) or absence (open circles) of 5 μM FK506. Data points shown are the mean ± S.E. from four separate experiments.