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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Anal Chem. 2009 Apr 1;81(7):2760–2767. doi: 10.1021/ac802707u

Figure 1.

Figure 1

Sensor schematic: (A) SAM formation on Au yields mixed monolayers of amine- and hydroxyl-terminated alkanethiols presenting the BoNT/A enzymatic substrate. (B) PDMS microchannels on 40 arrayed Au pads (10.5 mm2) (scale bar = 5 mm) with inset image representing two neighboring channels (scale bar = 1 mm). (C) BoNT/A is added at input port and incubated on SAMs, during which time it can cleave the immobilized substrate, releasing fluorescent fragments into solution. Flu-labeled fragments are concentrated at detection port via evaporation.