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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Anal Chem. 2009 Apr 1;81(7):2760–2767. doi: 10.1021/ac802707u

Figure 5.

Figure 5

BoNT/A light chain (50 µg/mL in HEPES) response to flu-SNAP SAMs at RT (blue ◇) and at 30 °C (blue ◆). ALC was able to cleave more flu-SNAP at elevated temperatures, producing fluorescent signals twice as intense as corresponding measurements at RT. The HEPES buffer control containing 1.3 mM DTT (black squares) produced a slight signal as a result of thiol exchange at the Au surface, whereas the HEPES control without DTT (red circles) produced negligible signals. Solid lines represent nonlinear fits to the data.