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Sensor output at varying ALC concentrations was quantified using fluorescence microscopy at the microchannel detection port. Normalized averages ±SE were plotted and fit to a nonlinear equation representing combined kinetic and surface adsorption concepts. The solid line under the curve represents the control (+1SE, dotted line). The sensor was able to detect down to 20 pg ALC per milliliter of buffered solution (all points, P < 0.05). Inset: 20 pg/mL−5 µg/mL, linear scale.
