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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Cancer Res. 2008 Sep 15;68(18):7352–7361. doi: 10.1158/0008-5472.CAN-07-6311

Figure 1. Expression of IG20 splice isoforms in human NB cell lines, primary NB tumor lines, and various human tissues.

Figure 1

1 μg of total RNA was used for reverse transcription-polymerase chain reaction (RT-PCR) using the Super-Script III OneStep RT-PCR system (Invitrogen Life Technologies, Carlsbad, CA, USA). A. Shows amplification of exon 34 region of IG20-SVs using F4824 and B5092 primers. B. Shows quantification of relative intensities of bands in relation to the housekeeping gene GAPDH from panel A using ImageJ (http://rsb.info.nih.gov/ij/).