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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Cancer Res. 2008 Sep 15;68(18):7352–7361. doi: 10.1158/0008-5472.CAN-07-6311

Figure 2. IG20-SVs and down modulation effect of exon-specific siRNAs directed against specific isoforms on endogenous IG20-SVs in SK-N-SH cells.

Figure 2

A. Shows human IG20-SVs generated by alternative mRNA splicing. Solid bars represent regions of complete cDNA sequence homology between variants. Empty areas indicate spliced exons 13L, 16, 21, 26 and 34, which when spliced in different combinations can give rise to the six IG20-SVs. B. Effect of down modulation of endogenous IG20-SVs by exon-specific siRNAs in SK-N-SH cells. One microgram total RNA obtained from GFP-positive SK-N-SH cells obtained by fluorescence-activated cell sorting (FACS) at 5 days post-transduction was used for reverse transcription–polymerase chain reaction. The products were separated on a 5% PAGE. Amplification of IG20-SVs using F1-B2 primers (upper panel) and F4824-B5092 (lower panel) is shown. C. Quantification of relative intensities of bands from panel B (upper panel) using ImageJ. D. Quantification of relative intensities of bands from panel B (lower panel) using ImageJ.