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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Cancer Res. 2008 Sep 15;68(18):7352–7361. doi: 10.1158/0008-5472.CAN-07-6311

Figure 4. Effects of TNF-α treatment on apoptosis of siRNA-transduced SK-N-SH cells.

Figure 4

Three days post-transduction, SK-N-SH cells were treated with 10ng/ml TNF-alpha for 2 days, and cells were collected and stained with Annexin V-PE/7-AAD. A. Summarized results showing percentage of cells with increased apoptosis from three independent experiments. The P-value was *P<0.05 for TNF-α treated cells vs untreated cells. B. Summarized results showing percentage of apoptosis in transduced NB cells, cells treated or un-treated with 10ng/ml TNFα after transfection with pcDNA or pcDNA-DN-FADD. Results are from three independent experiments. The P-value was *P<0.05, **P<0.01 for pcDNA-DN-FADD transfected cells vs pcDNA3.1 transfected cells. The data were collected from GFP-positive cells only.