Fig. 3.

Human aquaporin-1 expression in epithelial cells in vitro. Upper panel: (a) Northern blot using RNA from 293 cells transduced with either AdhAQP1 or a control vector, Adα1AT. (b) Western blot of crude membranes from 293 cells transduced with AdhAQP1 or the control vector. (c) Western blot of crude membranes from MDCK and SMIE cells transduced with AdhAQP1 or the control vector. Note that in the Western blots the monomeric non-glycosylated hAQP1 protein migrates at ∼28kDa, while multiple glycosylated forms are seen at slightly higher molecular weights. This figure originally was published as Fig. 1 in (Delporte et al. 1997). Lower panel: Localization of transgenic hAQP1 expressed in MDCK cells. Confluent MDCK cells were grown on filters and transduced for 24 h with either Adα1AT (a) and (b) or AdhAQP1 (c) and (d). Cell layers were then examined by confocal microscopy after immunofluorescent staining with an antibody to hAQP1. (a) and (c) are in the x–y plane, while (b) and (d) are in the x–z plane. This figure originally was published as Fig. 2 in (Delporte et al. 1997)