Table 1.
[69] Proteins found to be decreased or increased in the skin of high-fat fed diabetic mice following 16 weeks of feeding starting at 3 weeks of age as compared to protein level found in non-diabetic control mice fed standard rodent chow for 16 weeks starting at 3 weeks of age. Copyright Wiley-VCH Verlag GmbH & Co.KGaA. Reproduced with permission.
| Spot | Protein a | Mascot MS/MS Accession # | % Change(HF/C) b, c |
|---|---|---|---|
| B5 | creatine kinase (EC 2.7.3.2) chain M | A23590 | 936% ↓ |
| B10 | aldolase 1, A isoform | gi|42490830 | 612% ↓ |
| C7 | similar to glyceraldehyde-3-phosphate dehydrogenase | gi|6679937 | 805% ↓ |
| C12 | transferrin | gi|17046471 | 104% ↓ |
| G12 | creatine kinase (EC 2.7.3.2) chain M | A23590 | 127% ↓ |
| H8† | calpactin I light chain (Protein S100-A10) | S10AA_MOUSE | 192% ↓ |
| A12† | calpactin I light chain (Protein S100-A10) | S10AA_MOUSE | 153% ↑ |
| B3 | creatine kinase (EC 2.7.3.2) chain M | A23590 | 483% ↑ |
| B4 | creatine kinase (EC 2.7.3.2) chain M | A23590 | 413% ↑ |
| B6 | creatine kinase (EC 2.7.3.2) chain M | A23590 | 837% ↑ |
| C2 | 14-3-3 protein beta | gi|3065925 | 164% ↑ |
| C6 | peroxiredoxin 6 | gi|6671549 | 415% ↑ |
| C8 | phosphoglycerate mutase 1 | gi|10179944 | 208% ↑ |
| C11 | nucleoside-diphosphate kinase 2 | gi|6679078 | 350% ↑ |
| D1 | keratin complex 1, acidic gene 4 | gi|13386238 | 226% ↑ |
| D2 | apolipoprotein A-1 precursor | gi|109571 | 385% ↑ |
| D8 | lectin, galactose binding, soluble 7 | gi|31543120 | 120% ↑ |
| D9 | nucleoside-diphosphate kinase 1 | gi|37700232 | 63% ↑ |
| E1 | vacuolar protein sorting 29 | gi|9790285 | 64% ↑ |
| E4 | apolipoprotein E precursor | gi|114041 | 83% ↑ |
| E5 | prohibitin | gi|6679299 | 156% ↑ |
| E7 | malate dehydrogenase, cytoplasmic | gi|92087001 | 1262% ↑ |
| E12 | Keratin 1b | gi|38565071 | 808% ↑ |
| F1 | protein disulfide isomerase associated 3 | gi|112293264 | 1190% ↑ |
| F2 | proteasome (prosome, macropain) subunit, alpha type 1 | gi|33563282 | 195% ↑ |
| F4 | fatty acid-binding protein | PC4011 | 276% ↑ |
| F5 | fatty acid-binding protein, adipocyte | FABPA_MOUSE | 267% ↑ |
| F6 | fatty acid-binding protein, adipocyte | FABPA MOUSE | 403% ↑ |
A minimum of 2 significant MS/MS peptide fragments was considered sufficient to assign an ID for a spot. More detailed information can be found in the supplemental information section.
The mean decreases in spot intensities were determined using PDQuest 7.0.0 software on 24 gels from 8 mice.
Only differences that were statistically significant P<0.05 as determined by Student T-test as part of the PDQuest software are reported.
Indicates a weak identification by matching one significant fragment with at least one fragment that approached significance (P=0.014) & (P=0.089).