Sh3p8 is enriched in Schwann cells at paranodes. A, Immunostaining of rat sciatic nerves for sh3p8 and panNF. B, Immunostaining of rat sciatic nerves for ZO1 and panNF suggests sh3p8 is not a component of autotypic tight junctions. C, Retraction of Schwann cell membranes demonstrates paranodal sh3p8 is associated with Schwann cells rather than axons. D, Immunostaining of developing rat sciatic nerve for sh3p8 and the indicated node-associated proteins. Scale bars, 10 μm; the scale bar in A also applies to B.