Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Int J Parasitol. 2009 Jul 8;39(14):1561–1571. doi: 10.1016/j.ijpara.2009.06.005

Fig. 5.

Fig. 5

In vitro binding of cis-parinaric acid and retinol by recombinant Ancylostoma ceylanicum fatty acid and retinol binding protein-1 (rAceFAR-1). A) Fluorescence emission spectra (λexc = 319 nm) of 0.8 µM cis-parinaric acid alone or in combination with 3 µM rAceFAR-1. The displacement effect following addition of 10 µM oleic acid to the preformed cis-parinaric acid:rAceFAR-1 complex is also shown. B) Change in relative fluorescence intensity (λexc = 319 nm) of 1.0 µM rAceFAR-1 in the presence of increasing concentrations of cis-parinaric acid. The curve was used to derive the equilibrium dissociation constant, Kd, for the cis-parinaric acid:rAceFAR-1 interaction. C) Fluorescence emission spectra data (λexc = 350 nm) for rAceFAR-1 binding to 0.8 µM retinol. D) Similar analysis of binding affinity and Kd calculation for the retinol:rAceFAR-1 interaction.