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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Int J Parasitol. 2009 Jul 8;39(14):1561–1571. doi: 10.1016/j.ijpara.2009.06.005

Fig. 6.

Fig. 6

Ex vivo fatty acid uptake in adult Ancylostoma ceylanicm. A) Representative merged images of adult A. ceylanicum females (n = 3 per group) incubated with 5 µm 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ) and a 10-fold excess of various non-fluorescent fatty acids. B) Quantitation of the effect of co-incubation with excess unlabelled fatty acids on total fluorescent intensities of worms incubated with BODIPY FL C16. C) Effect of co-incubation of a 10-fold excess of the indicated fatty acids on the relative fluorescence intensity (λexc = 475 nm, λemm = 520 nm) of the recombinantAceFAR-1:BODIPYFLC16 complex (n = 3 for each fatty acid). C16, palmitic acid; C17, heptadecanoic acid; ARA, Arachidonic acid: EPA, Eicosapentaenoic acid; DHA, Docosahexaenoic acid; CHO, cholesterol. *indicates a statistically significant (P < 0.01) difference in mean signal intensity compared with the BODIPY FLC16 control group, as analyzed using ANOVA and Dunnett Multiple Comparison test.