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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Environ Mol Mutagen. 2009 Oct;50(8):718–724. doi: 10.1002/em.20509

Figure 4. Microtubule nucleation of CHO cell centrosomes.

Figure 4

CHO cells incubated with an anti-pericentrin antibody (green), an anti-β-tubulin antibody (red), and DAPI for DNA (blue). (Colocalization of pericentrin and β-tubulin appears yellow and colocalization of pericentrin, β-tubulin, and DAPI appears white.)

(A, B): Untreated CHO cells, without nocodazole incubation.

(C, D): 30 seconds after nocodazole removal. Centrosomes begin to recover microtubule aster formation.

(E, F): 5 minutes after nocodazole removal. Microtubule asters grow larger.

(G, H): 15 minutes after nocodazole removal. Normal microtubule distribution.

(I,J): 30 minutes after nocodazole removal. Cell division with multi-polar spindles.