Table II. Phosphorylated Peptides of E. coli Ribosomal Proteins Detected by LC-MS/MS Analysis.
| Start - End | Sequence | Phosphosite | XCorr | m/z | Mr(expt) | Mr(calc) | Delta |
|---|---|---|---|---|---|---|---|
| S3- 30S | |||||||
| 27-37 | EFADNLDSˆDFK | S34 | 2.04 | 647.67 | 1283.34 | 1284.26 | -0.92 |
| 114-125 | LVADSˆITSQLER | S118 (T120, S121) | 3.06 | 658.02 | 1314.04 | 1314.49 | 0.44 |
| S4- 30S | |||||||
| 26-43 | AIDT#KCKIEQAPGQHGAR | T29 | 2.66 | 668.37 | 2003.11 | 2004.15 | 1.04 |
| 128-145 | VVNIAS#YQVSPNDVVSIR | S133 (Y134, S137) | 3.62 | 1020.69 | 2040.38 | 2041.19 | 0.81 |
| 128-145 | VVNIA#SY#QVSPNDVVSIR | S133, Y134 (S137) | 2.72 | 1061.00 | 2121.00 | 2121.17 | 0.17 |
| 165-176 | EKPT#WLEVDAGK | T169 | 2.70 | 727.99 | 1454.96 | 1453.52 | -1.44 |
| 188-205 | S#DLSADINEHLIVELY#SK | S188, Y203 (S204) | 2.98 | 735.51 | 2204.51 | 2207.21 | 2.70 |
| S5- 30S | |||||||
| 29-44 | IFS#FTALTVVGDGNGR | S31 (T33) | 2.06 | 867.88 | 1734.75 | 1734.83 | 0.08 |
| 29-51 | IFS#FT#ALTVVGDGNGRVGFGY#GK | S31, T33 (T36), Y49 | 2.84 | 868.45 | 2603.35 | 2603.60 | 0.25 |
| 93-111 | VFMQPAS#EGTGIIAGGAM*R | S99 | 1.30 | 995.66 | 1989.31 | 1990.19 | -0.13 |
| S7-30S | |||||||
| 53-75 | SˆGKS#ELEAFEVALENVRPTVEVK | S53, S56 | 3.52 | 865.26 | 2594.78 | 2594.81 | 0.03 |
| 56-75 | S#ELEAFEVALENVRPTVEVK | S56 | 3.29 | 780.39 | 2339.15 | 2340.51 | 1.36 |
| 79-94 | VGGS#T#Y#QVPVEVR | S82, T83, Y84 | 2.35 | 816.51 | 1632.02 | 1631.49 | -0.53 |
| 79-97 | VGGS#T#Y#QVPVEVRPVR | S82, T83, Y84 | 3.19 | 662.46 | 1985.37 | 1983.93 | -1.44 |
| 111-136 | GDKS#M*ALRLANELSDAAENKGTAVK | S114 (S124) | 3.92 | 896.60 | 2687.78 | 2686.88 | -0.90 |
| 143-150 | MAEANKAFAHY#R | Y153 | 2.43 | 745.95 | 1490.88 | 1489.58 | 1.30 |
| S11-30S | |||||||
| 14-36 | QVS#DGVAHIHASFNNTIVTITDR | S16 (S25) | 2.78 | 859.29 | 2575.86 | 2576.70 | 0.83 |
| 53-68 | GS#RKS#T#PFAAQVAAER | S54, S57, T58 | 1.92 | 639.19 | 1915.56 | 1916.80 | 1.23 |
| 106-125 | ITNITDVT#PIPHNGCRPPK | T113 (T110) | 2.75 | 718.95 | 2154.83 | 2154.37 | 0.46 |
| 106-125 | ITNITDVTˆPIPHNGCRPPK | T113 (T110) | 2.69 | 1028.71 | 2056.37 | 2056.39 | -0.02 |
| S12- 30S | |||||||
| 36-50 | VY#TT#TPKKPNS#ALRK | Y37, T39 (T38), S45 | 2.18 | 972.55 | 1944.09 | 1944.93 | 0.85 |
| S13-30S | |||||||
| 44-56 | IS#ELSEGQIDTLR | S45 | 2.18 | 770.59 | 1540.18 | 1541.58 | 1.40 |
| S18- 30S | |||||||
| 30-42 | NY#IT#ESˆGKIVPSR | Y31, T33, S35 | 2.42 | 803.66 | 1606.31 | 1606.61 | 0.29 |
| L1-50S | |||||||
| 37-53 | FVES#VDVAVNLGIDARK | S40 | 2.65 | 956.54 | 1912.07 | 1913.06 | 0.99 |
| L2- 50S | |||||||
| 183-202 | VEADCRAT#LGEVGNAEHM*LR | T190 | 3.41 | 757.56 | 2270.67 | 2268.41 | -2.26 |
| L3- 50S | |||||||
| 14-33 | IFT#EDGVSIPVTVIEVEANR | T16 (S21, T25) | 3.65 | 757.04 | 2270.67 | 2269.43 | 0.33 |
| 14-33 | IFTˆEDGVS#IPVTVIEVEANR | T16 (S21), S21 (T25) | 3.96 | 751.41 | 2252.22 | 2251.43 | -0.79 |
| L5- 50S | |||||||
| 15-29 | LMT#EFNYNSVM*QVPR | T17 | 3.66 | 963.12 | 1925.24 | 1926.10 | 0.86 |
| 15-29 | LMT#EFNY#NSVM*QVPR | T17, Y21 (S23) | 3.38 | 669.19 | 2005.56 | 2006.08 | 0.52 |
| 125-147 | GNY#SM*GVREQIIFPEIDYDKVDR | Y127 or S128 | 2.58 | 948.57 | 2843.68 | 2842.03 | -1.65 |
| L6- 50S | |||||||
| 69-84 | ALLNS#MVIGVTEGFT#K | S73,T83 (T79) | 2.52 | 921.47 | 1841.93 | 1840.95 | -0.98 |
| L10-50S | |||||||
| 125-150 | LATLPTYEEAIARLM*ATMKEAS#AGK | S147 | 3.33 | 922.11 | 2764.31 | 2764.11 | -0.20 |
| L13- 50S | |||||||
| 14-23 | DWY#VVDATGK | Y16 | 2.06 | 616.83 | 1232.64 | 1234.23 | 1.59 |
| 39-60 | HKAEY#TˆPHVDTGDYIIVLNADK | Y44, T45 (T50) | 4.40 | 854.70 | 2562.08 | 2562.73 | 0.65 |
| 41-60 | AEY#TPHVDT#GDY#IIVLNADK | Y43 (T44), T49, Y53 | 3.01 | 825.71 | 2475.12 | 2475.37 | 0.25 |
| L14- 50S | |||||||
| 1-17 | M*IQEQTMLNVADNS#GAR | S14 (T6) | 2.73 | 664.62 | 1991.85 | 1991.09 | -0.76 |
| 109-122 | S#EKFMKIISLAPEVL | S109 (S117) | 2.58 | 596.68 | 1788.02 | 1786.06 | -1.96 |
| L16- 50S | |||||||
| 18-33 | GLAQGT#DVSFGSFGLK | T24 (S27) | 2.27 | 831.93 | 1662.85 | 1664.73 | 1.88 |
| 100-113 | VLY#EMDGVPEELAR | Y103 | 2.09 | 850.74 | 1700.48 | 1701.82 | 1.34 |
| 128-136 | TTFVT#KTVM | T132 (T134) | 1.53 | 554.77 | 1108.54 | 1108.23 | -0.31 |
| L18- 50S | |||||||
| 34-56 | HIYAQVIAPNGSˆEVLVAASTVEK | S45 (S52, T53) | 4.78 | 794.12 | 2380.37 | 2379.71 | -0.66 |
| L19- 50S | |||||||
| 14-28 | QDVPSˆFRPGDTVEVK | S18 | 3.09 | 829.02 | 1657.04 | 1656.84 | -0.20 |
| L21- 50S | |||||||
| 1-10 | M*Y#AVFQSGGK | Y2 | 2.01 | 592.77 | 1184.53 | 1184.24 | -0.29 |
| L22- 50S | |||||||
| 29-41 | VSQALDILT#Y#T#NK | T37, Y38, T39 | 2.39 | 854.25 | 1707.48 | 1706.60 | -0.88 |
| 100-110 | T#S#HIT#VVVSDR | T100, S101, T104 | 2.24 | 728.06 | 1455.10 | 1454.29 | -0.81 |
| L28- 50S | |||||||
| 4-18 | VCQVT#GKRPVTGNNR | T8 | 2.40 | 855.26 | 1709.51 1 | 709.8500 | 0.34 |
| L31- 50S | |||||||
| 9-23 | YEEITAS#CS#CGNVMK | S15 (T13), S17 (S15) | 2.91 | 899.22 | 1797.43 | 1795.83 | -1.60 |
| L31-B2- 50S | |||||||
| 34-52 | VIELDGVTYPYVTIDVS#SK | S50 (S51) | 2.47 | 1090.55 | 2178.60 | 2179.35 | -0.74 |
(ˆ) and (#) show the phosphorylated Ser(S), Thr (T), and Tyr(Y) residues. Residues in parenthesis show the other possible phosphorylation site(s) detected. (*) indicates the methionine oxidation. Mass spectrometry data was searched against the E. coli protein sequences in SwissProt database using Bioworks 3.2.