Table 1.
Expression vectors and transfected cell lines used in this study
| Name | Vectors |
|---|---|
| Vectors | |
| pflag (control) | pflag-CMV-1 (Kodak) (signal sequence followed by flag) |
| pflag/vzg-1 (experimental) | pflag-CMV-1 containing vzg-1 ORF |
| pcDNAI (control) | pcDNAI (Invitrogen) |
| pcDNAI/antisense vzg-1 (control) | pcDNAI containing antisense vzg-1 |
| pcDNAI/vzg-1 (experimental) | pcDNAI containing vzg-1 ORF |
| pHook2 (control) | pHook2 (Invitrogen) (phOx sFv, a marker protein co-expressed) |
| pHook2/vzg-1 (experimental) | pHook2 containing vzg-1 ORF |
| pBLCAT2* | |
| Stable cell lines | |
| E-7 (stable line for empty vector) | pcDNAI + pSV-neo |
| A-5 (stable line for antisense vzg-1) | pcDNAI/antisense vzg-1 + pSV-neo |
| S-3 (stable line for sense vzg-1) | pcDNAI/vzg-1 + pSV-neo |
| S-4 (stable line for sense vzg-1) | pcDNAI/vzg-1 + pSV-neo |
| S-7 (stable line for sense vzg-1) | pcDNAI/vzg-1 + pSV-neo |
| C-1 (stable line for CAT under SRE) | pBLCAT2 + pSV-neo |
*
From M. Karin, University of California, San Diego.