Fig. 6.

Double-label immunofluorescence microscopy of cryostat sections through bovine lens tissue presenting details of the cortex adhaerens. A comparison of the reaction for N-cadherin (N-cad, red) with that for the prominently gap-junction-associated protein ZO-1 (a, green), the actin-filament-associated protein ezrin (b, green), and the AJ plaque protein α-catenin (c, α-cat, green). Only the merged images are shown. Note that here the ZO-1 reaction appears to be restricted to a limited region in the longer lateral wall, whereas N-cadherin and α-catenin are highly enriched at junction-like structures in the short wall elements. Ezrin is seen in the entire cell cortex. For further details, see Straub et al. (2003). Bars 10 μm