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. 2009 Oct 22;4(10):e7468. doi: 10.1371/journal.pone.0007468

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Plaskon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Quantification of ONNV (−) strand RNA with a ssqPCR assay using unmodified primers (A), or a tagged primer system (B) produces significantly different values (P<0.001). Amplification of cDNAs generated in an unprimed RT reaction with unmodified ONNV-specific primers (A).