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. 2009 Oct 20;4(10):e7413. doi: 10.1371/journal.pone.0007413

Figure 2. NF-κB signaling drives C. jejuni induced cytokine gene expression in BMDC.

Figure 2

BMDC were generated from WT mice and treated with Bay 11-7085 (10 µM) and then stimulated with C. jejuni (MOI 50) for 4 h. Cells were lysed in Trizol, total RNA was extracted, reverse transcribed and IL-12p40 (A) and IL-6 (B) mRNA expression was detected using real-time PCR (Applied Biosystems 7700 sequence detection system). Results were normalized to the housekeeping gene GAPDH to ascertain similar loading. Results are the mean +/− SD of triplicates samples and are from one of three independent experiments (*p = 0.011 for IL-12p40 vs. Bay 11-7085-treated cells; *p = 0.005 for IL-6 vs. Bay 11-7085-treated cells).