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. 2009 Oct 20;4(10):e7413. doi: 10.1371/journal.pone.0007413

Figure 5. C. jejuni induces NF-κB signaling in IL-10−/−; NF-κBEGFP mice.

Figure 5

(A) C. jejuni induces NF-κB activity in the colon of IL-10−/−; NF-κBEGFP mice. IL-10wt/wt; NF-κBEGFP and IL-10−/−; NF-κBEGFP mice were colonized for 14 days with C. jejuni (108 cfu/mouse), mice were euthanized, colons dissected and EGFP expression immediately visualized using a CCD camera in a light-tight imaging box with a dual filtered light source. The data are representative of three independent experiments. IL-10wt/wt; NF-κBEGFP: n = 8, IL-10−/−; NF-κBEGFP: n = 4, IL-10wt/wt; NF-κBEGFP C. jejuni associated: n = 13, IL-10−/−; NF-κBEGFP C. jejuni associated: n = 12. (B) C. jejuni induces elevated EGFP protein expression in the colon. Colonic protein of C. jejuni associated IL-10wt/wt; NF-κBEGFPand IL-10−/−; NF-κBEGFP were extracted and 20 µg were used for SDS-PAGE gel analysis. Gels were stained with EGFP specific antibodies. n = 3 per group.