Skip to main content
. 2009 Oct 20;4(10):e7413. doi: 10.1371/journal.pone.0007413

Figure 7. Increased TNFα and IL-12p40 mRNA accumulation in C. jejuni-associated IL-10−/−; NF-κBEGFP mice.

Figure 7

IL-10wt/wt; NF-κBEGFP and IL-10−/−; NF-κBEGFP were associated with C. jejuni for 14 days. Colonic sections were lysed in trizol and RNA was extracted, reverse-transcribed (1 µg) and amplified by PCR using specific murine TNFa and IL12p40 primers. GAPDH was used as loading control. WT = IL-10wt/wt; NF-κBEGFP, n = 7; KO = IL-10−/−; NF-κBEGFP, n = 7; WT C. jejuni associated: n = 12; KO C. jejuni associated: n = 10. (*p = 0.002 for IL12p40 vs. control, *p = 0.001 for TNF vs. control).