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. 2009 Oct 27;7(10):e1000229. doi: 10.1371/journal.pbio.1000229

Figure 1. Pancreata of Tph1−/− mice have a normal morphology but lack 5-HT, which results in hyperglycemia and insulin resistance.

Figure 1

(A) 5-HT contents in Tph1−/− and wt mice show, that Tph1−/− pancreata contain only around 10% of the wt mean. *p<0.05; n = 6. (B) Combined stacks of confocal images of pancreas slices spanning 10 µm with anti-insulin-stained islets (green) and anti-S100-stained ductal structures (red) at postnatal day 3 (P3) reveal no difference between the genotypes; scale bar = 70 µm. (C) Individual islets do not differ in morphometric analysis (Tph1−/−: n = 29; wt: n = 30). (D) Basal blood glucose concentration in freely feeding Tph1−/− mice are significantly reduced at P3 (Tph1−/−: n = 20; wt: n = 11) and elevated at P14 (n = 5) and in adulthood (n = 6) compared to wt littermates. *p<0.05. (E) Glucose tolerance tests with fasted wt and Tph1−/− mice show impaired glucose tolerance of the latter. *p<0.05; n = 6. (F) Insulin tolerance tests with Tph1−/− and wt mice. *p<0.05; n = 6. For (A) and (C–F), data are means ± SEM.