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. 2009 Oct 16;4(10):e7495. doi: 10.1371/journal.pone.0007495

Figure 1. The reg4 gene is found with increased copy number in pancreatic cancer.

Figure 1

Allelic ratios were calculated with the Partek Genomics Suite, version 6.4. HapMap (270 samples) was used to create baseline copy number. Genomic segmentation was utilized to detect copy number gain or loss. Regions were detected using the following segmentation parameters: minimum of 10 genomic markers; segmentation p-value threshold lower than 0.001; a signal to noise equal to 0.3. A. Schematic representation of the reg4 locus. Positions of the 7 genes present in this locus are indicated: from left to righ: ZNF697, PHGDH, HMGCS2, REG4, NBPF7, ADAM30 and NOTCH2. B. Position of the copy number gain segment found in all 14 DNA samples from pancreatic cancer, which includes the reg4 gene. C. Genomic changes in the amplified segment of the reg4 locus, determined by the Affymetrix Genome-Wide Human SNP Array 6.0 analysis in the 14 pancreatic cancer samples. Genetic gains are shown as red bars and losses as blue bars, grey bars corresponding to 2 copies as indicated in the scale shown underneath. Note the predominance of gains (red) in the reg4 region. D. Detail of gains/losses in the reg4 gene and its flanking regions for all 14 analyzed samples.