Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Full-length extension of primer hybridized to modified or unmodified templates. Reactions were carried out at 30°C for 20 min using either a natural template (X = A, sequence 13, lanes 1, 2) or a modified template containing 3-NiA (X = 3-NiA, sequence 8, lanes 3 and 4) at a 100-nM concentration, primed with 32P-labelled 18-mer, sequence 9 (50 nM) in the presence of the four dNTPs (at a final concentration of 20 μM each). Reactions were catalysed by KF exo- (0.1 U).
