Figure 4.

Transcription factor and co-factor recruitment at the huβ-globin locus in ln2 and Δ2B e12.5 EryC. (A–D) ChIP assays were carried out on e12.5 fetal liver EryC (e12.5; black bars: ln2; dotted bars: Δ2B. Immunoprecipitated and input chromatin samples were subject to qPCR. Fold enrichments were calculated as described in Figure 1 and are indicated on the y-axis. β (βmaj) control replaces mHS2 control for analysis in fetal liver cells. Hash sign (#): P ≤ 0.05 according to Student's; t-test (ln2 versus Δ2B). The regions analyzed are specified on each graph and the antibodies used for ChIP assays are indicated underneath each graph.