Figure 1.

Representative plots of the kinetics of protection. Increase in fractional saturation at different sites on the promoter on both the template and non template strands for the three conditions studied here, wt T7A1 at 37°C, wt T7A1 at 20°C and the consensus –10 mutant T7A1 promoter at 37°C. The goodness to fit to either a single or double exponential were determined by a visual analysis of the residuals and an F-test analysis (see Supplementary Table 2 online and Supplementary Figure 4 online for the complete set of the plots and the analysis of the residuals).