Table 1.
Nucleotide Position | Apparent rate (s−1) |
Percentage of total amplitude |
||
---|---|---|---|---|
kA | kB | A | B | |
Wild-type T7A1 promoter at 37°C | ||||
m12 | 0.6 ± 0.2 | 0.049 ± 0.009 | 45 | 55 |
m10 | 0.7 ± 0.2 | 0.046 ± 0.008 | 47 | 53 |
m6 | 0.8 ± 0.2 | 0.036 ± 0.006 | 47 | 53 |
m4 | 0.35 ± 0.07 | 0.025 ± 0.004 | 49 | 51 |
p1 | 1.0 ± 0.2 | 0.019 ± 0.004 | 52 | 48 |
Consensus –10 T7A1 promoter at 37°C | ||||
m12 | 0.8 ± 0.3 | 0.025 ± 0.006 | 37 | 63 |
m4 | 0.7 ± 0.2 | 0.018 ± 0.006 | 48 | 52 |