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The new RNase III cleavage band detected in 1–570 RNA transcript (lanes 1–2) is indicated by X, and is shown to be highly promoted in a 5′ truncated fragment ∼36–570 (lanes 3–4). Lanes 1 and 3 are 1–570 and ∼36–570 RNA transcripts incubated in buffer, and lanes 2 and 4 are the same transcripts incubated with 0.001 U/μl of RNase III.
