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. 2009 Jul 3;37(16):5498–5510. doi: 10.1093/nar/gkp553

Figure 8.

Figure 8.

Analysis of induced conformational changes of 1–570 RNA on non-denaturing gel electrophoresis. (A) 1–570 RNA was resuspended at 0.6 nM in each of the following conditions: in water and kept on ice (lane 1), in standard buffer reaction alone (lanes 2 and 6), together with ODN 22(−) at a final concentration of 15 nM, 150 nM and 1500 nM (lanes 3–5), or with miR-122 minus seed sequence at increasing final concentrations of 1.5 nM, 15 nM or 150 nM (lanes 8–10). Lane 7, control ODN 22(−) at 1500 nM. (B) Ice-incubated control RNA is in lane 1, and buffer-incubated RNA controls are in lanes 2 and 7. RNA was incubated together with miR-122 at a final concentration of 1.5 nM, 15 nM and 150 nM (lanes 3–5). Lane 6, control 22(−) or together with seed sequence (5′ U GGAGUGU GA 3′) at concentrations of 1.5 nM, 15 nM and 150 nM (lanes 8–10). ‘ORI’ marks the origin of the electrophoresis. Arrows marked with ‘O’ and ‘C’ indicate open and close conformations respectively.