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Expression of the ecl18kI genes in vivo. RNA was purified from E. coli cells harboring plasmids with ecl18kI.M (A) or ecl18kI.R (B) promoters fused to promotorless galK in the absence (lanes 1) or presence (lanes 2) of a compatible plasmid expressing M.Ecl18kI and subjected to primer extension with a galK specific primer.
