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. 2009 Jul 10;37(16):5322–5330. doi: 10.1093/nar/gkp579

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — The time course of accumulation of full-sized ecl18kI transcripts in vitro. Escherichia coli RNAP σ⁷⁰ holoenzyme was combined with DNA fragments containing the indicated promoters and a single round of transcription was initiated by the addition of NTPs and heparin. PCR fragments used as templates for in vitro transcription allowed the generation of similarly sized full-sized transcripts from both ecl18kI promoters. Reaction aliquots were withdrawn and transcription terminated at times indicated. Reaction products were resolved by denaturing PAGE and revealed by autoradiography.