Table 1.

Representative total RNA quantity and quality from microdissected frozen tissue samples ^a.

Tissue	Neoplastic Status	# LCM Shots	Total RNA Quantity (ng/ul)	Total RNA Quality (RINb)
Epithelium:			Range = 8.4 – 20.42	Range = 4.2 – 7.8
Breast	Normal	2000	12.29	7.6
	Tumor	3000	13.51	7.8
Colon	Normal	3000	13.2	4.2
	Tumor	3000	12.57	6.5
Esophagus	Normal	4000	16.59	5.1
	Tumor	3700	20.42	4.5
Prostate	Normal	3000	8.4	6.2
	Tumor	3000	10.8	5.6
Urethra	Normal	2000	13.87	6.1
Stroma:			Range = 4.81 – 8.86	Range = 4.8 – 6.9
Breast	Normal	3000	6.53	6.8
	Tumor	2400	8.86	6.5
Prostate	Normal	20,000	6.65	6.9
	Tumor	20,000	6.37	5.7
Urethra	Normal	3000	4.81	4.8

^aThis data was generated at the Pathogenetics Unit, NCI and is representative of frozen microdissected total RNA from these tissues.

^bRIN = RNA Integrity Number. Algorithm assigning value to RNA quality, as calculated by Bioanalyzer 2100 (Agilent, Inc.).