Dilution	Total RNA concentration	Recipe
1:1	1000.0 ng/μl	100 μl of 1000.0 ng/μl total RNA
1:5	200.0 ng/μl	20 μl of 1:1 in 80 μl of H2O
1:10	100.0 ng/μl	10 μl of 1:1 in 90 μl of H2O
1:20	50.0 ng/μl	5 μl of 1:1 in 95 μl of H2O
1:50	20.0 ng/μl	10 μl of 1:5 in 90 μl of H2O
1:100	10.0 ng/μl	10 μl of 1:10 in 90 μl of H2O
1:200	5.0 ng/μl	10 μl of 1:20 in 90 μl of H2O
1:500	2.0 ng/μl	10 μl of 1:50 in 90 μl of H2O