| Reagent | Volume to add per reaction |
|---|---|
| 10x Buffer | 5.00 μl |
| MgCl2 | 11.00 μl |
| 10mM dNTP | 10.00 μl |
| RNase Inhibitor | 1.00 μl |
| Random Hexamers | 2.50 μl |
| Multiscribe RT Enzyme | 1.25 μl |
| H2O | 15.25 μl |
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