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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Am J Transplant. 2009 Jul 28;9(10):2251–2261. doi: 10.1111/j.1600-6143.2009.02786.x

Figure 4. bm12 heart allografts in CCR5-/- recipients have more infiltrating CD4 T cells and fewer Tregs as compared to grafts from wild-type recipients.

Figure 4

A. bm12 allografts were harvested from wild-type B6 or CCR5-deficient recipients on day 10 post-transplant and following digestion and antibody staining, infiltrating cells were analyzed by flow cytometry for CCR5 and FoxP3 expression. Plots shown are representative of 5 mice per group and 2 separate experiments. B. bm12 allografts were harvested from wild-type or CCR5-/- recipients on day 10 post-transplant. Infiltrating cells were quantified by flow cytometry as described. Data represents mean ± SEM, n = 4-5/group. C. bm12 heart allografts were retrieved from wild-type B6 or B6.CCR5-/- recipients on day 10 post-transplant and mRNA prepared from graft tissue homogenates was subjected to quantitative RT-PCR analysis for FoxP3 and IL-10 expression. Samples from groups of 5 recipients were plated in triplicate. Data represents mean ± SEM; ND = none detected.