Figure 1.

An overview of potential outcomes of GSV integration into the genome. The left panels demonstrate how integration of the Gene Search Vector in an "antisense orientation" would disrupt Allele 1 and then facilitate overexpression of an antisense to disrupt the second allele in the presence of inducer (RSL1). On the other hand, the right panels demonstrate how integration in a sense orientation would facilitate overexpression of the target gene (or domains thereof). Note that the phenotype is tightly regulated by the RSL1-inducible promoter, thus allowing the investigation to reverse the phenotype by turning the GSV vector "off" when cells are cultured in the absence of RSL1.