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V-gene usage and VH CDR3 length of unselected and antigen-specific scFv. The VH and VL genes were sequenced and the germ-line gene was assigned based on homology to a database (vbase) of germ-line V-genes compiled by Tomlinson et al. (25). Specific VH, Vκ, and Vλ genes are listed on the ordinate, with the VH, Vκ, or Vλ germ-line gene family indicated below. Only V-genes in unselected or selected clones are listed.
