Table 2.
Affinities and binding kinetics of anti-BoNT A C-fragment and anti-Erb-B2 scFv
| Specificity and clone | Kd (× 10−9 M) | kon (× 105 M−1s−1) | koff (× 10−3 s−1) |
|---|---|---|---|
| ErbB-2 B7A | 0.22 | 4.42 | 0.1 |
| ErbB-2 G11D | 0.48 | 2.19 | 0.11 |
| ErbB-2 A11A | 0.49 | 3.69 | 0.18 |
| ErbB-2 F5A | 4.03 | 1.62 | 0.65 |
| BoNT-A 2A9 | 26.1 | 0.25 | 0.66 |
| BoNT-A 2H6 | 38.6 | 2.2 | 8.5 |
| BoNT-A 3F6 | 66.0 | 4.7 | 30.9 |
| BoNT-A 2B6 | 71.5 | 1.1 | 7.8 |
Association (kon) and dissociation (koff) rate constants for purified scFv were measured by using surface plasmon resonance (BIAcore) and Kd was calculated as (koff/kon).