Table 3.
Comparison of protein binding antibodies selected from nonimmune phage-display antibody libraries
| Library | Library size and type* | Number of protein antigens studied | Average number of antibodies per protein antigen | Number of affinities measured | Range of affinities for protein antigens Kd (×10−9 M) |
|---|---|---|---|---|---|
| Marks et al. (12) | 3.0 × 107 (scFv, N) | 2 | 2.5 | 1 | 100–2000 |
| Nissim et al. (13) | 1.0 × 108 (scFv, SS) | 15 | 2.6 | ND | ND |
| deKruif et al. (42) | 3.6 × 108 (scFv, SS) | 12 | 1.9 | 3 | 100–2,500 |
| Griffiths et al. (14) | 6.5 × 1010 (Fab, SS) | 30 | 4.8 | 3 | 7.0–58 |
| Vaughan et al. (15) | 1.4 × 1010 (scFv, N) | 3 | 7.0 | 3 | 4.2–8.0 |
| Sheets et al. (this work) | 6.7 × 109 (scFv, N) | 14 | 8.7 | 8 | 0.22–71.5 |
*
For library type, N = V-gene repertoires obtained from V-genes rearranged in vivo; SS = semisynthetic V-genes constructed from cloned V-gene segments and synthetic oligonucleotides encoding VH CDR3. ND, not determined.