Table 1.
SSP | L:Ha | Protein symbol | Protein full name | gi | Matched peptides | Sequence coverage (%) |
---|---|---|---|---|---|---|
5719 | 0.71 | GPD2 | Glycerol-3-phosphate dehydrogenase | 4504085 | 14 | 24 |
610 | 0.52 | P4HB | Prolyl 4-hydroxylase, β subunit precursor | 20070125 | 12 | 39 |
5726b | 0.73 | WDR1 | WD repeat domain 1 | 12652891 | 14 | 28 |
3105b | 0.78 | PGLS | 6-Phosphogluconolactonase | 6912586 | 6 | 26 |
102 | 0.77 | PSMA5 | Proteasome (prosome, macropain) subunit, α type, 5 | 7106387 | 7 | 39 |
5716 | 0.63 | ATIC | 5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase | 20127454 | 14 | 27 |
5627 | 0.70 | OXCT1 | OXCT | 48146215 | 8 | 28 |
6406b | 1.54 | GSN | GSN isoform b | 38044288 | 18 | 24 |
5207 | 0.79 | PNP | Purine nucleoside phosphorylase | 108935929 | 18 | 58 |
8102b | 0.61 | TLN1 | talin 1 | 16753233 | 4 | 2 |
4613 | 0.75 | CCT2 | Chaperonin containing TCP1, subunit 2 | 5453603 | 9 | 25 |
201 | 0.63 | TPM4 | Tropomyosin 4 | 4507651 | 11 | 61 |
2303 | 0.81 | TUBA1B | TUBA1B protein | 18204869 | 6 | 28 |
9313b | 0.73 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 31645 | 14 | 35 |
4408 | 0.72 | ENO1 | Enolase 1 | 4503571 | 7 | 21 |
5405 | 0.72 | Unknown | Actin-related protein | 381964 | 7 | 23 |
4009 | 0.68 | RAB7B | RAB7B protein | 16877705 | 5 | 45 |
7203 | 0.74 | MGLL | Monoglyceride lipase isoform 2 | 51242953 | 12 | 55 |
2208 | 0.65 | ACTG1 | γ-actin | 178045 | 4 | 28 |
1303 | 0.76 | U2AF1 | U2 small nuclear RNA auxiliary factor 1 | 31455228 | 6 | 42 |
4007 | 0.85 | PARK7 | DJ-1 protein | 31543380 | 11 | 50 |
7003 | 0.83 | PSMA2 | PSMA2 protein | 39644890 | 7 | 31 |
4010 | 0.80 | PRDX3 | Peroxiredoxin 3 isoform b | 32483377 | 6 | 17 |
1001 | 0.68 | Unknown | T669 kinase | 1362991 | 14 | 28 |
6104b | 0.82 | TPI1 | Chain B, triosephosphate isomerase (Tim) (E.C.5.3.1.1) complexed with 2-phosphoglycolic acid | 999893 | 9 | 41 |
3705b | 0.80 | VCL | Vinculin | 24657579 | 8 | 8 |
106 | 0.70 | PLEK | Pleckstrin | 4505879 | 5 | 15 |
8404 | 0.79 | Unknown | Chain, aldolase A | 229674 | 10 | 42 |
7213 | 0.79 | FGA | Fibrinogen α chain preproprotein, isoform α-E | 13591824 | 7 | 7 |
2304 | 0.79 | TUBA6 | Tubulin α 6 | 14389309 | 7 | 24 |
8505 | 1.12 | FGB | Fibrin β | 223002 | 15 | 51 |
5401 | 0.74 | PKM2 | Pyruvate kinase, muscle | 31416989 | 6 | 19 |
7105b | 1.32 | RSU1 | RSU1 protein | 14250499 | 13 | 65 |
7115 | 0.85 | CA2 | Chain, carbonic anhydrase Ii (E.C.4.2.1.1) mutant with Pro 202 replaced by Ala (P202a) | 443135 | 11 | 51 |
9405 | 1.05 | IDH2 | Isocitrate dehydrogenase 2 (NADP+), mitochondrial precursor | 28178832 | 9 | 23 |
6203b | 0.91 | CAP1 | Adenyly 1 cyclase-associated protein | 5453595 | 6 | 22 |
8301 | 1.08 | ANXA2 | Annexin A2 | 18645167 | 7 | 28 |
7006 | 1.06 | SOD2 | SOD2 | 30841309 | 6 | 27 |
3002c | 0.83 | GPX1 | Glutathione peroxidase | 14717805 | 1 | 61 |
All the proteins were identified with a MALDI-TOF/TOF Ultraflex mass spectrometer, and the quality of match was evaluated at the significance level (p<0.05) for peptide fingerprint mass spectra and/or peptide sequencing spectra; SSP: the spot identity on the 2-DE gel designated by the PDQuest; gi: the accession number of proteins in NCBI protein database.
The ratio of mean normalized values for the corresponding protein spot in low BMD group to high BMD group.
The spot with the highest MASCOT score and/or with most significant differential expression among multiple spots of the same protein.
An exceptional protein, for which the differential expression is statistically insignificant; it showed 20% higher expression in high BMD group than in low BMD group (L:H = 0.83).