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. Author manuscript; available in PMC: 2009 Oct 13.
Published in final edited form as: Clin Chem. 2009 Feb 20;55(4):757–764. doi: 10.1373/clinchem.2008.116467

Table 1.

qRT-PCR detection of melanoma markers in serially diluted melanoma cell lines (n = 4).a

Cell number

Marker 104 103 102 101 100 0

GAPDH 4 4 4 4 4 0

MLANA 4 4 4 4 4 0

MAGEA3 4 4 4 4 4 0

MITF 4 4 4 4 3 0
a

Data represent the number of times the indicated marker was detected (of 4 experiments) at the indicated numbers of melanoma cells mixed with 5 × 106 PBCs. An HMW-MAA mAb mixture was used in an indirect assay with immunomagnetic beads. GAPDH is a housekeeping gene used as an internal control.