Table 2.

Dye/protein starting ratios and calculated dye/protein ratios for listed conjugates

Conjugate	Dye/IgG starting ratio, mol/mol	Dye/IgG ratio in the conjugate, mol/mol*	Yield per IgG, %*	Dye/IgG ratio in the conjugate, mol/mol**	Yield per IgG, %**
Seta635-IgG	15	5.1	82	13.7	47
Seta670-IgG1	2.5	0.4	33	0.5	33
Seta670-IgG2	5	0.9	70	1.2	68
Seta670-IgG3	10	2.2	92	3.2	86
Seta670-IgG4	15	3.1	93	6.5	79
Seta670-IgG5	23	4.7	57	10.9	44

^*calculated using dye’s absorption peak height (at 638–642 nm).

^**calculated using dye’s absorption peak area (integrated from 500 to 750 nm).