Table 4.

Multiexponential analysis of the fluorescence intensity decays of Seta dyes

Compound/conditions	<τ>*, ns	τ̄**, ns	α1	τ1*, ns	α2	τ2*, ns	χR2
Seta670-IgG4 conjugate on glass	0.57	1.04	0.67	0.19	0.33	1.29	0.9
Seta670-IgG4 conjugate on SIFs	0.06	0.09	0.96	0.048	0.04	0.25	2.1
Seta635-IgG conjugate on glass	0.67	1.29	0.71	0.26	0.29	1.67	0.9
Seta635-IgG conjugate on SIFs	0.09	0.15	0.91	0.068	0.09	0.32	1.3

^* $<\tau >={\displaystyle \sum _i}{\alpha }_i{\tau }_i$

^** $\overline{\tau }={\displaystyle \sum _i}{f}_i{\tau }_i$, f_i = α_iτ_i/(Σα_iτ_i)