Table 1.

Comparison of Fixed Tissue Proteomic Studies, organized chronologically

	CELL COUNT	GRAMS PROTEIN	BUFFER	Mass Spectrometer	PEPTIDES	PROTEINS	PROTEINS ID’D BY MULT PEPTIDES
Hood ’05 - Cancer	200,000 cells	Not noted	Liquid Tissue	either a linear ion trap (LTQ, Thermo Electron, San Jose, CA) or a hybrid LIT-FT- ICR MS.	>2200	1156	282
Crockett ‘05	10,000,000 cells	600ug	RIPA	LCQ Deca XP ion trap (Thermo Electron, San Jose, CA, USA)	10,043	324	324
Palmer -Toy ‘05	Not noted	Not noted	2% SDS/100mM NaHCO3/ 20mM DTT	LCQ DECA XP plus Proteome X workstation (Thermo Electron Corporation, San Jose, CA).	412	123	42
Shi ‘06	Not noted	1.67 ug/uL	20 mM Tris- HCl buffer (pH 7 or 9) w/2%SDS	LTQ (Thermo Finnigan San Jose, CA)	4074 (avg, 2 samples)	3259 (avg, 2 samples)	1896
Hwang ‘07	675,000 cells (cells in 10 spots ×6 × 2 arrays)	82 ug	100mM ammonium bicarbonate	LTQ-linear ion- trap, Finnigan	12,631	428	361
Guo ‘07	100,000 cells	Not noted	20mM Tris, 2% SDS	LTQ linear ion trap (LTQ, Thermo- Finnigan; San Jose, CA)	11,398	2265	Not noted
Jiang ‘07	Not noted	10.9 ug/uL	40mM Tris, 6 M guanidine- HCl, and 65 mM DTT, pH 8.2	LTQ linear ion trap (Thermo, San Jose, CA)	3005	827	470
Patel ‘08 — WD HNSCC	80,000 cells per lesion type	Not noted	Liquid Tissue	LTQ linear ion trap (Thermo Electron, San Jose, CA)	1400-1800	323-376	323-376