Figure 3.

Yeast Cbf5p exhibits high sequence similarity to other pseudouridine synthases. Regions directly adjacent to the catalytic aspartate residue are strictly conserved within the TruB family of pseudouridine synthases. Grey shaded boxes represent amino acids which are strictly conserved or conservative amino acid substitutions with respect to its column. Black underlined letters (only apparent in the TruB of E. coli row) represent a non-similar amino acid with respect to that column.